mouse anti α adaptin Search Results


mouse monoclonal anti-adaptin γ (88  (Becton Dickinson)
90
Becton Dickinson mouse monoclonal anti-adaptin γ (88
Mouse Monoclonal Anti Adaptin γ (88, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+monoclonal+anti+adaptin+%CE%B3++88/pmc11103404-194-45-51
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mouse monoclonal anti-adaptin γ (88 - by Bioz Stars, 2026-09
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mouse anti-adaptin α  (Becton Dickinson)
90
Becton Dickinson mouse anti-adaptin α
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti Adaptin α, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+anti+adaptin+%CE%B1/pmc05785209-367-5-9
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mouse anti-adaptin α - by Bioz Stars, 2026-09
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mouse anti-γ-adaptin  (Becton Dickinson)
90
Becton Dickinson mouse anti-γ-adaptin
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti γ Adaptin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+anti+adaptin+%CE%B3/pmc06396150-584-58-62
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mouse anti-γ-adaptin - by Bioz Stars, 2026-09
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mouse anti-ap1γ1  (Becton Dickinson)
90
Becton Dickinson mouse anti-ap1γ1
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti Ap1γ1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+monoclonal+anti+ap+1%CE%B31+88++adaptin+%CE%B3/bio_rxiv__2020__02__12__945162-262-4-6
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mouse anti-ap1γ1 - by Bioz Stars, 2026-09
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mouse anti-ap1 (γ-adaptin) (610386)  (Becton Dickinson)
90
Becton Dickinson mouse anti-ap1 (γ-adaptin) (610386)
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti Ap1 (γ Adaptin) (610386), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+anti+ap1++%CE%B3+adaptin+++610386+/pmc05386352-136-0-18
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mouse anti-ap1 (γ-adaptin) (610386) - by Bioz Stars, 2026-09
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mouse anti-c-adaptin  (Becton Dickinson)
90
Becton Dickinson mouse anti-c-adaptin
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti C Adaptin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+anti+c+adaptin/pm26168397-296-34-36
Average 90 stars, based on 1 article reviews
mouse anti-c-adaptin - by Bioz Stars, 2026-09
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monoclonal mouse anti-adaptin 8  (Becton Dickinson)
90
Becton Dickinson monoclonal mouse anti-adaptin 8
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Monoclonal Mouse Anti Adaptin 8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/monoclonal+mouse+anti+adaptin+8/pm28799243-206-9-14
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monoclonal mouse anti-adaptin 8 - by Bioz Stars, 2026-09
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purified mouse anti-adaptin alpha for ap-2 alpha subunit  (Becton Dickinson)
90
Becton Dickinson purified mouse anti-adaptin alpha for ap-2 alpha subunit
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Purified Mouse Anti Adaptin Alpha For Ap 2 Alpha Subunit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/purified+mouse+anti+adaptin+alpha+for+ap+2+alpha+subunit/pmc04383858-223-21-24
Average 90 stars, based on 1 article reviews
purified mouse anti-adaptin alpha for ap-2 alpha subunit - by Bioz Stars, 2026-09
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mouse anti adaptin ƴ (ap 1  (Becton Dickinson)
90
Becton Dickinson mouse anti adaptin ƴ (ap 1
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti Adaptin ƴ (Ap 1, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+anti+adaptin+%C6%B4++ap+1/pmc07196918-270-157-161
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mouse anti adaptin ƴ (ap 1 - by Bioz Stars, 2026-09
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mouse anti-adaptin alpha 8  (Becton Dickinson)
90
Becton Dickinson mouse anti-adaptin alpha 8
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti Adaptin Alpha 8, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+anti+adaptin+alpha+8/pmc09182567__ppat__1010252__s013-0-141-146
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mouse anti-adaptin alpha 8 - by Bioz Stars, 2026-09
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mouse anti-g-adaptin (610385)  (Becton Dickinson)
90
Becton Dickinson mouse anti-g-adaptin (610385)
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti G Adaptin (610385), supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+%CE%B1+adaptin/mouse+anti+g+adaptin++610385+/10__1096_slash_fj__201601052r-37-0-11
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mouse anti-adaptin β (ap2  (Becton Dickinson)
90
Becton Dickinson mouse anti-adaptin β (ap2
( A ) FRAP analysis of GFP-tagged AP2 α <t>adaptin</t> (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.
Mouse Anti Adaptin β (Ap2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) FRAP analysis of GFP-tagged AP2 α adaptin (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.

Journal: eLife

Article Title: NECAPs are negative regulators of the AP2 clathrin adaptor complex

doi: 10.7554/eLife.32242

Figure Lengend Snippet: ( A ) FRAP analysis of GFP-tagged AP2 α adaptin (APA-2:GFP) on membranes of coelomocytes (top schematic). Time constants (tau) of the fluorescence recovery are plotted. ( B ) AP2 localization in coelomocytes. Representative confocal images of coelomocytes in worms expressing APA-2:GFP. Micrographs (top) are representative maximum projections of Z-slices through approximately half of a cell. Data represent the coefficient of variance (%CV) of pixel intensities for individual cells. ( C ) Artificial AP2 cargo assay. Representative confocal micrographs of intestinal cells (middle) in worms expressing a GFP-tagged cargo (top schematic). TM, transmembrane domain. The average pixel intensity along a basolateral membrane was measured (bottom). ( A–C ) Bars indicate mean ±SEM for n ≥ 8 biological replicates. *p<0.05, **p<0.001, not significant (n.s.), unpaired, two-tailed T-test. ( D ) µ2 protease-sensitivity assay. Western blot analysis of whole worm lysates was used to quantify the amount of full-length µ2 (anti-HA, 50 kDa) before (pre TEV, bottom blot) and after protease induction (post TEV, top blot). Band intensities were compared to a tubulin loading control and normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( E ) µ2 phosphorylation assay. Western blot analysis of whole worm lysates to quantify phosphorylated µ2 (top blot) relative to total µ2 subunit (bottom blot). Values indicate band intensity ratios of phospho µ2 compared to total µ2, normalized to the fcho-1 (+) ncap-1 (+) ratio (values below). ( D and E ) Blots are representative of ≥3 biological replicates. +, wild type allele; -, deletion allele.

Article Snippet: Primary antibodies and dilutions included mouse anti-adaptin α (1:500, BD Biosciences, San Jose, CA, 610501), rabbit anti-AP2B1 (1:1000, Abcam, Cambridge, MA, 151961), rabbit anti-AP2M1 phospho T156 (1:1000, Abcam 109397), rabbit anti-AP2S1 (1:4000, Abcam 128950), mouse anti-flag (1:1000, Sigma-Aldrich F3165), mouse anti-tubulin (1:2000, Sigma-Aldrich T5168), rabbit anti-histone H3 (1:4000, Abcam 1791), and rabbit anti-beta actin (1:1000, Abcam, 8227).

Techniques: Fluorescence, Expressing, Two Tailed Test, Sensitive Assay, Western Blot, Phosphorylation Assay